Retinoids’ Extraordinary Potential

Monday, August 28, 2017

This story first appeared on the Cornell Research site. Read the original here.

Vitamin A enables some undeniably essential functions in the human body: the workings of the immune system, vision, and cell growth. In the early 1980s, researchers discovered that vitamin A and one of its metabolites, retinoic acid, also perform a critical function that could help treat cancers.

Researchers found that retinoic acid induces stem cells to differentiate into functional cells—muscle or skin cells, for example. In leukemia, high doses of retinoic acid caused differentiation of stem cells into functional blood cells, helping to successfully treat patients.

These studies piqued the interest of Lorraine J. Gudas, Pharmacology, Weill Cornell Medicine. The leukemia discovery was so remarkable because it shed new light on cancer. While people knew cancer was a disease of uncontrolled cell proliferation, the leukemia study made researchers appreciate that cancer cells often keep reproducing because they don’t receive the proper signals to differentiate.

“They’re stuck in that stem cell state and can’t differentiate to become a functional cell,” Gudas says. “We thought this was really fascinating and could lead to treatments for a number of diseases.”

Gudas has been probing the pharmacological potential of retinoids—the broader group of metabolites and derivatives of vitamin A—ever since. What she and her collaborators have found has illuminated the process of stem cell differentiation and opened new avenues for treating cancers. Recently, to Gudas’ surprise and delight, she and her team made breakthroughs in understanding retinoids’ seemingly critical role in diabetes.

Diabetes, Obesity, and Vitamin A Deficiency

Lorraine Gudas, Ph.D., in her lab Gudas’ newest area of research began when her lab used retinoic acid to induce the differentiation of embryonic stem cells into pancreatic endocrine cells. These are cells in the pancreas that produce insulin, the same cells that die in patients with diabetes.

If the body needs retinoic acid to make insulin-producing cells, what happens if the body doesn’t have enough retinoic acid? Is diabetes associated with vitamin A deficiency?

To test this idea Gudas and her lab put mice models on a vitamin A deficient diet. Within 10 weeks, the mice models manifested a type of advanced diabetes. “We showed that the vitamin A deficiency essentially led to the loss of cells that make insulin in the pancreas,” Gudas says. “No one had ever shown this before.”

Diabetes is on the rise in developing countries, where food and nutrients may be scarce. Gudas speculates that the diabetes in these countries may be due to a vitamin A deficiency. Gudas’ next question was about patients in the United States and other developed countries with obesity-associated diabetes, who would seem to have plenty of vitamin A in their diets.

Gudas’ team put mice models on a high fat diet that contained normal levels of vitamin A. The mice models as expected, developed obesity and diabetes. When they tested the blood of the models, they found normal levels of vitamin A. Then Gudas—along with former postdoctorate Steven Trasino, now Assistant Professor at Hunter College, and Weill Cornell Medicine faculty colleague Xiao-Han Tang, Pharmacology—tested the tissues.

“What we found, and this was a big shock to us, was that the obese, diabetic mice, despite having normal vitamin A levels in their diet and their blood, had very little vitamin A in their tissues, including the liver, lungs, kidneys, and pancreas,” Gudas says. “We guessed that just as in the non-obese mice, vitamin A deficiency in the tissues of the obese mice models was associated with damage to the pancreatic cells that make insulin.”

This vitamin A deficiency in the tissues is still a puzzle. “Is the vitamin A reaching the organs and then coming out quickly? Is it just not getting into the organs, getting stored somewhere else in the body, or being excreted?” Gudas asks. “This is something we need to understand, but we’re excited because we are certain this discovery has implications for human health.”

The next step will be understanding the mechanism by which localized vitamin A deprivation is occurring and to test whether the results in mice models match aspects of the disease in humans. The initial findings, however, constitute a breakthrough. In addition, Gudas notes, “Fatty liver disease, also on the rise in America and also associated with obesity, could be linked to vitamin A deficiency in the liver as well.” Further studies have indicated this is true.

“Sometimes research can go on slowly for quite some time, and it’s not super exciting every day,” Gudas says. “You just keep working, moving step by step. But when a result turns out to be unexpected, and then you can go on to make and prove your hypothesis, it’s very energizing.”

Cancer and Synthetic Retinoids

Lorraine Gudas, Ph.D.Lorraine Gudas, Ph.D. Cancer has been the main focus of Gudas’ work over the past 20 years, and she and her lab continue to make strides alongside their newer projects. They have described the mechanisms by which retinoids initiate stem cell differentiation, shedding light on how changes in signaling can have vastly different outcomes in terms of how differentiated cells form. They have also developed mouse models of various cancers—head and neck and kidney—testing different retinoids on the tumors themselves.

One cancer type that shows great potential for treatment with retinoids are epithelial cancers—cancers of the surface tissues, such as skin cells or the cells of the tongue or the lining of the throat. Even though retinoic acid is used clinically to treat leukemias, it isn’t the perfect weapon against these epithelial cancers; the retinoic acid gets metabolized too fast and doesn’t select specifically for epithelial cells. In a recent discovery, Gudas and her team found a synthetic retinoid that better fits the bill.

About 12 years ago, Gudas and Tang, then a postdoctorate, developed a mouse model that manifests oral cavity and esophageal squamous cell carcinoma, a cancer that forms from epithelial stem cells. Recently, they treated the models with a synthetic retinoid, called CD-1530, and found that the drug greatly inhibited cancer formation. One of the drug’s features is that it selectively binds to only one of three receptors for retinoic acid, a receptor that is highly expressed in epithelial cells. Therefore, the drug is better able to target those cells. In addition, the mice model metabolizes CD-1530 more slowly, giving the drug time to do its work.

“This drug had never been used before in a squamous cell carcinoma model like this,” Gudas says. “We have to do more animal tests and toxicology studies, but we would like to develop this drug for use in humans.”

Gudas is also optimistic about other synthetic retinoids that can similarly target specific receptor proteins. Gudas and Tang are founders with equity in Sveikatal Inc., a company developing drug candidates to treat diseases, such as diabetes, fatty liver disease, and cancer. They and Trasino are named on related patents developed at Cornell.

“I’ve been working with retinoids and vitamin A for a number of years,” Gudas says, “and you think that you know a lot about the field, and I certainly do. But then we keep making these surprising discoveries that I wouldn’t have predicted 10 or 15 years ago. So I’m ready to keep going. I think there are many new discoveries to be made.”

A Love of Solving Scientific Puzzles

Although Gudas has been chairman of the Pharmacology Department at Weill Cornell Medicine for more than 20 years, she still feels like a graduate student. “I love coming to work every day on my bike, as I did in grad school!” she says.

While she has always wanted to work on research problems that could benefit patients, she says the puzzle and problem solving—the scientific process—is a reward in and of itself. That appreciation has helped her in her leadership role.

“I get excited about the research going on in many labs, I really do, because I can see the scientific puzzles they’re trying to solve and how important they are,” she says. “I can get stimulated by the research being carried out by the faculty and students in our department and in the entire medical school. Every place has a feel, a culture, and the culture at Weill Cornell Medicine is very supportive, interactive, and friendly. Everyone here is trying to make the school better by doing the most innovative research we can.”